Journal: Arthritis research & therapy

Article Title: Both T and B cells are indispensable for the development of a PBMC transfer-induced humanized mouse model for SSc.

doi: 10.1186/s13075-022-02896-6

Figure Lengend Snippet: Fig. 5 Effect of immunosuppressive therapies in patients on disease manifestations in mice. Relative amounts of various lymphocyte subsets in PBMC of SSc patients treated with or without immunosuppressive (IS) drugs. The percentages of CD3+, CD4+, and CD8+ T cells as well as of CD20+ B cells in freshly isolated PBMC were detected by FACS before transfer into recipient mice (A). Comparison of severity of inflammation in the lungs (B) and kidneys (C), human IgG (D), survival curves (E), and mortality rates (F) between mice that received PBMC from patients treated with immunosuppressive (IS) drugs and those that received PBMC from patients without IS drugs. p values for comparisons in A–D were calculated using the Mann U test, and those indicated in E and F were calculated using the log-rank (Mantel-Cox) test and Fisher’s exact test, respectively

Article Snippet: To deplete T or B cells, 2 × 107 human PBMC were incubated with anti-human CD3 microbeads (Miltenyibiotec) or anti-human CD19 microbeads (Miltenyibiotec), respectively, using the AutoMACS program.

Techniques: Isolation, Comparison

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Integrated multiomic analysis identifies TRIP13 as a mediator of alveolar epithelial type II cell dysfunction in idiopathic pulmonary fibrosis

doi: 10.1016/j.bbadis.2024.167572

Figure Lengend Snippet: Key resources.

Article Snippet: Mouse IgG1 anti-human CD326 (EpCAM)-conjugated microbeads , Miltenyi Biotec , Cat# 130–061–101 RRID: AB_2832928.

Techniques: Plasmid Preparation, Magnetic Beads, Recombinant, Blocking Assay, Lysis, Protease Inhibitor, Red Blood Cell Lysis, Software, Microscopy, Fluorescence, Imaging

Journal: Journal of visualized experiments : JoVE

Article Title: Retroviral overexpression of CXCR4 on murine B-1a cells and adoptive transfer for targeted B-1a cell migration to the bone marrow and IgM production

doi: 10.3791/61003

Figure Lengend Snippet: Table of Materials

Article Snippet: Incubate on ice for 10 minutes at 4 °C. table ft1 table-wrap mode="anchored" t5 caption a7 Name of Material/Equipment Company Catalogue Number Comments/Description anti-CD16/CD32, or Fc block Life Technologies MFCR00 Ter119 biotin eBioscience 13–5921–82 Clone: Ter119 CD3e biotin eBioscience 13–0033–85 Clone: eBio500A2 Gr-1 biotin eBioscience 13–5931–82 Clone: RB6–8C5 CD23 biotin eBioscience 13–0232–81 Clone: B3B4 NK1.1 biotin BD Biosciences 553163 Clone: PK136 F4/80 biotin Life Technologies MF48015 Clone: BM8 anti-biotin microbeads Miltenyi Biotec 130–090–485 LS columns Miltenyi Biotec 130–042–401 CD5 PE eBioscience 12–0051–83 Clone: 53–7.3 IgM PECF594 BD Biosciences 562565 Clone: R6–60.2 CD23 PECy7 eBioscience 25–0232–82 Clone: B3B4 B220 APC eBioscience 17–0452–83 Clone: RA3–6B2 CD19 APCef780 eBioscience 47–0193–82 Clone: eBio1D3 CD45.1 PerCP-Cy5.5 BD Biosciences 560580 Clone: A20 CD45.2 BV421 BD Biosciences 562895 Clone: 104 CXCR4 APC eBioscience 17–9991–82 Clone: 2B11 Live/Dead Yellow Life Technologies {"type":"entrez-nucleotide","attrs":{"text":"L34968","term_id":"522211","term_text":"L34968"}} L34968 RPMI-1640 Gibco 11875–093 heat-inactivated fetal bovine serum Gibco 16000–044 HEPES Gibco 15630–080 Non-essential amino acids Gibco 11140–050 Sodium pyruvate Gibco 11360–070 Gentamicin Gibco 15710–064 Beta-mercaptoethanol Gibco 21985–023 ODN 1668 InvivoGen tlrl-1668 70 micron filter caps Falcon 352235 Insulin syringes BD Biosciences 329461 Ctl-GFP retrovirus N/A N/A Generated in house using GFP-expressing retroviral plasmid MigR1 provided by Dr. T.P.

Techniques: Blocking Assay, Generated, Retroviral, Plasmid Preparation, Cloning, Software